Lineage and phylogenetic analysis of HPV-16, -18 in saliva of HNSCC patients

Aim: Head and Neck Squamous Cell Carcinoma (HNSCC) is a global health problem whose incidence varies by geographic region race according to risk factors. Human papillomavirus (HPV) infection significant factor for HNSCC. HPV-16 HPV-18 are two forms of HPV that carcinogenic. HNSCCs positive have better prognosis rather than negative. The purpose this research was characterize HPV-16, -18 variations in the saliva HNSCC patients examining genetic diversity utilizing full E6, E7, L1 genes. Methods: case-control included 15 with healthy volunteers. Unstimulated entire samples were obtained from case control groups spitting method. Genomic DNA isolated all samples. A PCR reaction used determine presence saliva. HPV-positive genotyped data analyzed. We conducted variant study on E7 Results: Three HPVpositive genotypes out 30 people diagnosed HPV-DNA. most common genotypes. genes sequenced compared (E6, E7) prototype sequence. In all, lineages A1 A3 discovered. Conclusion: Regarding variation found Iranian patients, need further studies genotyping seen. Sequencing may help answer questions about population. genotype analysis aids development vaccinations against HNSCC, halting disease progression preventing HPV-associated